Pepstatine ni pentapeptide, ibisanzwe bisanzwe bya aspartyl protease inhibitor, ishobora kubuza protease aspartic na protease ya protease ya mikorobe itandukanye.Pepstatine isohorwa cyane nubwoko bwa Streptomyces kandi ikorwa na Streptomyces.Irashobora kubuza pepsin, pepsin D na enzyme irekura angiotensin, kandi ikagira ingaruka zo kuvura ibisebe byo mu gifu, hypertension yimpyiko, arthritis, karrageenan edema nizindi ndwara.
Pepstatine ninzitizi ikomeye ya protease ya aspartyl nka pepsin, cathepsin D, na renin.Iyi pentapeptide karemano yitandukanije na actinomycetes yari imaze imyaka myinshi inhibitor ya renin inhibitor muri vitro.Pepstatine ntabwo yihariye renin kandi ntishobora gushonga mumazi.Ibikomoka ku miterere ya pepstatine byongereye imbaraga kandi byihariye kuri renin byateganijwe nubunini bwinshi. kuri inzibacyuho ya peptide bond hydrolysis na protease protease.
Iyo poroteyine ikuwe mu ngirabuzimafatizo zacitse, protease zirashobora kurekurwa, zigomba guhagarikwa byihuse kugira ngo poroteyine itangirika.Muburyo bwo gukuramo poroteyine, inhibitor za protease zigomba kongerwaho kugirango wirinde proteolysis.Inzitizi ya poroteyine yerekeza cyane cyane ku kintu gihuza amatsinda amwe ku kigo gikora cya molekile ya protease, ku buryo ibikorwa bya protease bigabanuka cyangwa bikabura, ariko ntibisobanura poroteyine ya enzyme.Ibyiyumvo bya poroteyine zitandukanye kuri poroteyine zitandukanye biratandukanye, birakenewe rero guhindura ubunini bwa poroteyine zitandukanye.Kuberako imbaraga za protease inhibitor mumazi ari muke cyane, hakwiye kwitabwaho byumwihariko ko protease inhibitor igomba kuvangwa byuzuye muri buffer kugirango igabanye imvura ya protease inhibitor.Pepstantine A irashobora kubuza protease aside nka pepsin, angiotensin, kolagenase, cathepsin D na chymosine.
Pepstatin A ni inhibitori ya cathepsin d na e.Nyuma ya selile HEK293 imaze kuvurwa hamwe na pepstatine A itandukanye yamasaha 24, hamenyekanye imvugo ya LC3Ⅱ na p62.Ibisubizo byagaragaje ko pepstatine A ishobora kuzamura cyane imvugo ya LC3Ⅱ na P62 (P.<0.05) muburyo bukabije.20μ g / ml pepstatine A yakoreshejwe mu kuvura HEK293 mugihe gitandukanye, kandi ingaruka zigihe cyigihe zitandukanye kumvugo ya LC3II na p62 zaragaragaye.Ibisubizo byerekanaga ko pepstatine A ishobora kugenzura imikorere ya LC3II na p62 muburyo bushingiye kumwanya.
Turi polypeptide mu Bushinwa, dufite uburambe bwimyaka myinshi mukubyara umusaruro wa polypeptide.Hangzhou Taijia Biotech Co., Ltd. ni uruganda rukora ibikoresho bya polypeptide yabigize umwuga, rushobora gutanga ibihumbi icumi by’ibikoresho fatizo bya polypeptide kandi birashobora no gutegurwa ukurikije ibikenewe.Ubwiza bwibicuruzwa bya polypeptide nibyiza, kandi ubuziranenge bushobora kugera kuri 98%, byamenyekanye nabakoresha kwisi yose. Murakaza neza kutugisha inama.
