GsMTx4 ni peptide ya 35-amino acide ifite imigozi ine ya disulfide ikora motif ya sisitemu, ikaba ari ibintu bisanzwe biranga imiterere ya peptide yubumara bwigitagangurirwa itanga ituze kandi yihariye.Uburyo bwibikorwa bya GsMTx4 ntabwo bwasobanuwe neza, ariko byizerwa ko buhuza na domaine zidasanzwe cyangwa transembrane domaine ya MSC cationic kandi ikabuza gufungura imyenge cyangwa amarembo ihindura ihinduka ryabo cyangwa impagarara.GsMTx4 yerekanwe kubuza MSCs cationic nyinshi guhitamo hamwe nimbaraga zitandukanye.Kurugero, GsMTx4 ibuza TRPC1 hamwe na IC50 ya 0.5 μM, TRPC6 hamwe na IC50 ya 0.2 μM, Piezo1 hamwe na IC50 ya 0.8 μM, Piezo2 hamwe na IC50 ya 0.3 μM, ariko nta ngaruka igira kuri TRPV1 cyangwa TRPV4 yibitekerezo bigera kuri 10 μM.(Bae C et al 2011, Ibinyabuzima)
GsMTx4 yakoreshejwe nkigikoresho cya farumasi cyo kwiga imikorere nogutunganya MSCs cationic mubwoko butandukanye bwimitsi.Zimwe mu ngero ni:
GsMTx4 irashobora guhagarika MSCs ikora mukurambura astrocytes, selile yumutima, ingirabuzimafatizo zoroheje hamwe na selile skeletal.Astrocytes ni selile zimeze nkinyenyeri zifasha ubwonko nu mugongo.Ingirabuzimafatizo z'umutima ni selile zigize imitsi y'umutima.Utugingo ngengabuzima tworoheje ni selile zigenzura urujya n'uruza rw'ingingo nk'igifu n'amaraso.Utugingo ngengabuzima twa skeletal ni selile zifasha kugenda kubushake bwumubiri.Muguhagarika MSCs muri selile, GsMTx4 irashobora guhindura imiterere yumuriro wamashanyarazi, urwego rwa calcium, kugabanuka no kuruhuka, hamwe nimvugo ya gene.Izi mpinduka zirashobora kugira ingaruka kuburyo izo selile zikora mubisanzwe cyangwa mubihe byindwara (Suchyna et al., Kamere 2004; Bae et al., Biochemie 2011; Ranade et al., Neuron 2015; Xiao et al., Nature Chemical Biology 2011)
GsMTx4 irashobora kandi guhagarika ubwoko bwihariye bwa MSC bwitwa TACAN, bugira uruhare mubisubizo byububabare.TACAN ni umuyoboro ugaragarira mu ngirabuzimafatizo zumva ububabare.TACAN ikoreshwa nubukanishi, nkumuvuduko cyangwa gukubita, kandi bigatera ububabare.GsMTx4 irashobora kugabanya ibikorwa bya TACAN no kugabanya imyitwarire yububabare mubyitegererezo byinyamaswa zububabare bwa mashini (Wetzel et al., Nature Neuroscience 2007; Eijkelkamp et al., Itumanaho rya Kamere 2013)
GsMTx4 irashobora kurinda astrocytes uburozi buterwa na molekile yitwa lysophosphatidylcholine (LPC), ikaba umuhuza wa lipide utera kwangiza ubwonko nu mugongo.LPC irashobora gukora MSCs muri astrocytes ikanabatera gufata calcium nyinshi, biganisha ku guhagarika umutima no gupfa.GsMTx4 irashobora kubuza LPC gukora MSCs muri astrocytes no kubarinda uburozi.GsMTx4 irashobora kandi kugabanya kwangirika kwubwonko no kunoza imikorere yubwonko bwimbeba zatewe na LPC (Gottlieb et al., Journal of Biological Chemistry 2008; Zhang et al., Journal of Neurochemistry 2019)
GsMTx4 irashobora guhindura imitekerereze itandukanye ya selile mu guhagarika ubwoko bwihariye bwa MSC bwitwa Piezo1, bugaragarira mu ngirabuzimafatizo.Ingirabuzimafatizo ngengabuzima ni selile zishobora gukora neuron nshya cyangwa ubundi bwoko bwingirabuzimafatizo.Piezo1 ni umuyoboro ukoreshwa nubushakashatsi bwibidukikije buturuka ku bidukikije, nko gukomera cyangwa umuvuduko, kandi bigira ingaruka ku buryo ingirabuzimafatizo zikomeye zifata ubwoko bw'utugingo ngengabuzima.GsMTx4 irashobora kubangamira ibikorwa bya Piezo1 no guhindura itandukaniro ryimyanya myakura itandukanye ya neuron ikagera kuri astrocytes (Pathak et al., Journal of Cell Science 2014; Lou et al., Raporo z'akagari 2016)
Turi polypeptide mu Bushinwa, dufite uburambe bwimyaka myinshi mukubyara umusaruro wa polypeptide.Hangzhou Taijia Biotech Co., Ltd. ni uruganda rukora ibikoresho bya polypeptide yabigize umwuga, rushobora gutanga ibihumbi icumi by’ibikoresho fatizo bya polypeptide kandi birashobora no gutegurwa ukurikije ibikenewe.Ubwiza bwibicuruzwa bya polypeptide nibyiza, kandi ubuziranenge bushobora kugera kuri 98%, byamenyekanye nabakoresha kwisi yose. Murakaza neza kutugisha inama.
